Immunotherapy treatments continue to dominate the biopharmaceutical industry, representing a significant effort in drug discovery. Characterizing the intracellular signaling pathways that govern the activity of immune cell subsets is crucial to advance our understanding of the role of the immune system in disease and potential therapeutic mechanisms. Flow cytometry has emerged as the gold standard for identifying novel aspects of immune cell function and has the unique ability to measure intracellular signaling events at a single-cell level while providing necessary immune cell phenotyping information.
Additionally, flow cytometry techniques often examine extracellular markers to phenotype immune cells, but investigating intracellular proteins with flow requires a modified approach. Although such experiments might appear challenging at first, it is easier than you may think. In this webinar, we will discuss the feasibility of using flow to reveal essential signaling insights needed to uncover the activity and function of rare immune cell subsets, as well as show how to optimize flow fixation/permeabilization protocols for multiplexed antibody panels—to simultaneously detect essential post-translational signaling modifications in immune cells and vital immunophenotyping information.