| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| SET1A (D3V9S) Rabbit mAb | 61702 | 20 µl | 300 kDa | Rabbit IgG |
| SET1B (D1U5D) Rabbit mAb | 44922 | 20 µl | 320 kDa | Rabbit IgG |
| MLL1 (D2M7U) Rabbit mAb (Amino-terminal Antigen) | 14689 | 20 µl | 300 kDa | Rabbit IgG |
| MLL1 (D6G8N) Rabbit mAb (Carboxy-terminal Antigen) | 14197 | 20 µl | 180 kDa | Rabbit IgG |
| MLL2/KMT2B (D6X2E) Rabbit mAb (Carboxy-terminal Antigen) | 63735 | 20 µl | 80 kDa | Rabbit IgG |
| WDR5 (D9E1I) Rabbit mAb | 13105 | 20 µl | 37 kDa | Rabbit IgG |
| WDR82 (D2I3B) Rabbit mAb | 99715 | 20 µl | 30 kDa | Rabbit IgG |
| Menin (D45B1) XP® Rabbit mAb | 6891 | 20 µl | 68 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The SET1/COMPASS Antibody Sampler Kit provides an economical means of detecting SET1/COMPASS proteins using control antibodies against SET1A, SET1B, MLL1, MLL2, WDR5, WDR82, and Menin. This kit contains enough primary antibodies to perform at least two western blot experiments.
Storage
Background
The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 on lysine 4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3 and MLL4, all of which methylate histone H3 on lysine 4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common core structural subunits WDR5, RBBP5 and ASH2L (2-6). WDR82 is a core subunit specific to SET1A and SET1B complexes, while Menin is a core subunit specific to the MLL complexes (4,5,7).
Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 on lysine 4 (2-6). SET1A, SET1B, MLL1 and MLL2 mediate di- and tri-methylation of histone H3 Lys4 at gene promoters to facilitate transcription activation. MLL3 and MLL4 function primarily to mono-methylate histone H3 Lys4 at gene enhancers. MLL1 and MLL2 function as master regulators of both embryogenesis and hematopoiesis, and are required for proper expression of Hox genes (8-10). MLL1 is a large approximately 4000 amino acid protein that is cleaved by the Taspase 1 threonine endopeptidase to form N-terminal (MLL1-N) and C-terminal MLL1 (MLL1-C) fragments, both of which are subunits of the functional MLL1/COMPASS complex (11,12). MLL1 translocations are found in a large number of hematological malignancies, suggesting that Set1 histone methyltransferase complexes play a critical role in leukemogenesis (6). Like MLL1, MLL2 is also a large, approximately 2700 amino acid protein that is cleaved by the Taspase 1 threonine endopeptidase to form N-terminal (MLL2-N) and C-terminal (MLL2-C) fragments, both of which are subunits of the functional MLL2/COMPASS complex. MLL2 has also been implicated as a modulator of hematological malignancies (13). MLL3 and MLL4 proteins are not cleaved by Taspase 1.
- Miller, T. et al. (2001) Proc Natl Acad Sci U S A 98, 12902-7.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
- Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.
- Yokoyama, A. et al. (2004) Mol Cell Biol 24, 5639-49.
- Eissenberg, J.C. and Shilatifard, A. (2010) Dev Biol 339, 240-9.
- Smith, E. et al. (2011) Genes Dev 25, 661-72.
- Denissov, S. et al. (2014) Development 141, 526-37.
- Takeda, S. et al. (2006) Genes Dev 20, 2397-409.
- Yokoyama, A. et al. (2002) Blood 100, 3710-8.
- Chen, Y. et al. (2017) Cancer Cell 31, 755-770.e6.
Background References
Trademarks and Patents
Limited Uses
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