Cannabinoid Receptor 1 Downstream Signaling Antibody Sampler Kit (#93521) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Mol. Wt	Isotype/Source
CB1 Receptor (D5N5C) Rabbit mAb	93815	20 µl	60 kDa	Rabbit IgG
Phospho-CREB (Ser133) (87G3) Rabbit mAb	9198	20 µl	43 kDa	Rabbit IgG
CREB (48H2) Rabbit mAb	9197	20 µl	43 kDa	Rabbit IgG
Phospho-Akt (Ser473) (D9E) XP^® Rabbit mAb	4060	20 µl	60 kDa	Rabbit IgG
Akt (pan) (C67E7) Rabbit mAb	4691	20 µl	60 kDa	Rabbit IgG
Phospho-mTOR (Ser2448) (D9C2) XP^® Rabbit mAb	5536	20 µl	289 kDa	Rabbit IgG
mTOR (7C10) Rabbit mAb	2983	20 µl	289 kDa	Rabbit IgG
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP^® Rabbit mAb	4370	20 µl	44, 42 kDa	Rabbit IgG
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb	4668	20 µl	46, 54 kDa	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody	7074	100 µl		Goat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Cannabinoid Receptor 1 Downstream Signaling Antibody Sampler Kit provides an economical means of detecting the activation of downstream cannabinoid receptor signaling pathways using phospho-specific and control antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

Cannabinoid receptors mediate a number of physiological processes in the brain ranging from appetite regulation, pain, learning, and memory (1). The major cannabinoid receptors in the brain include CB1 and CB2 receptors, which are G-protein coupled receptors (GPCRs). CB1 interacts with other GPCRs including metabotropic glutamate receptor 1, mGluR1 (2). Endogenous ligands, endocannabinoids, but also exogenously introduced compounds such as tetrahydrocannabinol (THC), activate cannabinoid receptors by promoting the exchange of GDP for GTP, leading to a cascade of signaling pathways that are activated to drive various functions. Some of these functions include neurite outgrowth, inflammation, and transcriptional control (3). Components of this kit are readouts for several downstream signaling components of CB1 receptor and they can also be used as a readout for CB1 activation and function. Cannabinoid receptor function is not limited to brain function but may modulate peripheral functions, including immune responses (4,5).

Background References

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.