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64839
A-769662
Activators & Inhibitors
Chemical Modulators

A-769662 #64839

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other Image 1 - A-769662

Western blot analysis of extracts from PC-3 cells, untreated (-) or treated with A-769662 (1 mM, 1hr; +), using Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 (upper) or AMPKα Antibody #2532 (lower).

other Image 2 - A-769662

Chemical structure of A-769662.

Product Usage Information

A-769662 is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 924.9 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 360.4 g/mol
Purity

>98%

Molecular Formula

C20H12N2O3S

CAS 844499-71-4
Solubility Soluble in DMSO at 30 mg/ml or ethanol at 5 mg/ml with slight warming.

Background

A-769662 is a potent activator of AMP-activated protein kinase (AMPK) by allosterically binding to the β and γ subunits and by inhibiting dephosphorylation of AMPK on Thr172 (1). This small molecule selectively targets AMPK heterotrimers containing the β1 subunit (2). A-769662 has been shown to stimulate purified rat liver AMPK (EC50 = 0.8 µM) and inhibit fatty acid synthesis in rat hepatocytes (IC50 = 3.2 µM) (3). Treatment of adipocytes with A-769662 inhibits cell differentiation, leads to over-activation of AMPK, and inactivation of acetyl-CoA carboxylase (ACC), making this an interesting compound when studying obesity (4). 

  1. Sanders, M.J. et al. (2007) J Biol Chem 282, 32539-48.
  2. Scott, J.W. et al. (2008) Chem Biol 15, 1220-30.
  3. Cool, B. et al. (2006) Cell Metab 3, 403-16.
  4. Zhou, Y. et al. (2009) Biol Pharm Bull 32, 993-8.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 64839S
Product # Size Price
64839S
5 mg N/A

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