Chemical structure of Bromosporine.
Bromosporine is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.23 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Molecular Weight: 404.4 g/mol
Molecular Formula: C17H20N6O4S
Solubility: Soluble in DMSO at 25 mg/ml.
Bromosporine is a potent broad-spectrum bromodomain inhibitor (1). Bromodomain and extra-terminal (BET) protein family members BRD2, BRD3, BRD4, and BRDT associate with acetylated lysines on histones and transcription factors to regulate their function, and have emerged as targets for cancer therapy (2,3). Treatment with Bromosporine induced the expression of latent HIV-1 in primary CD4+ T cells by inhibiting BET activity, leading to improved viral clearance in conjunction with antiretroviral therapy (4).
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