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9973
Cyclosporin A
Activators & Inhibitors
Chemical Modulators

Cyclosporin A #9973

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Cyclosporin A: Image 1

Dose response inhibition of IL-2 by Cyclosporin A in Jurkat cells treated overnight with TPA (40 nM) and A23187 (2 μM).

Cyclosporin A: Image 2

Structure of Cyclosporin A

Product Usage Information

Cyclosporin A is supplied as a 100 mg powder. Store at -20ºC. Cyclosporin A is soluble in ethanol at 200 mg/ml (also DMSO at 100 mg/ml) and stock solutions should be stored at -20ºC. Working concentrations and length of treatment can vary depending on the desired effect but it is typically used at around 100 nM.

Storage

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 1202.61 g/mol
Purity

>99%

Molecular Formula

C62H111N11O12

CAS 59865-13-3
Solubility Soluble in DMSO at 100mg/ml and EtOH at 200mg/ml.

Background

The calcium dependent protein phosphatase calcineurin is responsible for the de-phosphorylation of the transcriptional regulator nuclear factor of activated T cells (NFAT) and is essential for NFAT’s nuclear translocation and activation (1,2). Calcineurin is a target of two common immunosuppressants, cyclosporin A (CsA) (3) and FK-506 (also know as tacrolimus and fugimycin) (4), both of which can inhibit antigen and mitogen triggered T cell activation. These drugs interact with the immunophilins cyclophilin and FKBP-12, respectively, and the immunophilin-drug complex binds to calcineurin to inhibit substrate binding (5). FK-506 can be up to 100-fold more potent than CsA in various models (6-8).

  1. Schulz, R.A. and Yutzey, K.E. (2004) Dev. Biol. 266, 1-16.
  2. Rusnak, F. and Mertz, P. (2000) Physiol. Rev. 80, 1483-521.
  3. Borel, J.F. et al. (1976) Agents Actions 6, 468-75.
  4. Kino, T. et al. (1987) J Antibiot (Tokyo) 40, 1249-55.
  5. Liu, J. et al. (1991) Cell 66, 807-15.
  6. Henderson, D.J. et al. (1991) Immunology 73, 316-21.
  7. Tocci, M.J. et al. (1989) J Immunol 143, 718-26.
  8. Yoshimura, N. et al. (1989) Transplantation 47, 351-6.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 9973S
Product # Size Price
9973S
100 mg N/A

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