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Activators & Inhibitors

Oligomycin #9996

Western Blotting

Western blot analysis of extracts from 293 cells, untreated (-) or treated with Oligomycin (5 µM, 30 min; +), using Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 (upper) or AMPKα Antibody #2532 (lower).

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Oligomycin is supplied as a lyophilized powder. For a 5 mM stock, reconstitute the 5 mg in 1.26 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 0.5 - 10 μM for 0.5 - 8 hours.


Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Molecular Weight: 792.3 g/mol

(Average based on isomer content)



Molecular Formula:

Isomer A: C45H74O11

Isomer B: C45H72O12

Isomer C: C45H74O10

During oxidative phosphorylation in mitochondria, the electron transport system pumps H+ from the matrix into the intermembrane compartment. This process generates a H+ gradient across the mitochondrial inner membrane. This H+ gradient is used to drive mitochondrial FoF1 ATPase to convert ADP to ATP. Oligomycin, an antibiotic from Streptomyces diastatochromogenes, blocks oxidative phosphorylation by binding to the Fo part of the ATPase to inhibit its activity (1-3).

  1. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2461-6.
  2. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2467-74.
  3. Fessenden, J.M. and Racker, E. (1966) J Biol Chem 241, 2483-9.
For Research Use Only. Not For Use In Diagnostic Procedures.

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