|M||Endogenous||Rat IgG2a, kappa|
Flow cytometric analysis of live mouse splenocytes, untreated (left column) or treated with LPS #14011 (500 ng/ml, 3 d; right column), using CD86/B7-2 (GL-1) Rat mAb (PE Conjugate) (top row) or concentration-matched Rat Isotype Control (PE Conjugate) (bottom row), and co-stained with CD19 (ID3) Rat mAb (APC Conjugate) #39831.Learn more about how we get our images.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
posted June 2017
Protocol Id: 1504
For optimal flow cytometry results, we recommend 0.125 μg of antibody per test.
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
CD86/B7-2 (GL-1) Rat mAb (PE Conjugate) recognizes endogenous levels of total CD86/B7-2 protein. This antibody detects an epitope within the extracellular domain.
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
This Cell Signaling Technology antibody is conjugated to PE and tested in-house for direct flow cytometric analysis in mouse cells.
CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9).
The GL-1 antibody is used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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|60712S||100 µg (10 western blots)||N/A|
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