|H M R Mk||Endogenous||Rabbit IgG|
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Anisomycin #2222 (green), using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (PE Conjugate).Learn more about how we get our images.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
posted July 2009
revised June 2017
Protocol Id: 407
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (PE Conjugate) detects endogenous levels of ATF-2 only when phosphorylated at Thr71. The unconjugated antibody does not cross-react with phosphorylated c-Jun, CREB, or other transcription factors, but it does recognize ATF-2 dually phosphorylated at Thr69/Thr71 and singly phosphorylated at Thr71 equally well.
Human, Mouse, Rat, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF-2 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb #5112.
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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|13850S||100 µl (50 tests)|
75-77 Knowl Piece Wilbury Way
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