Western blot analysis of nonradioactive kinase assay performed to test efficacy of ATP (10mM) #9804. NIH-3T3 cells serum starved overnight and exposed to UV (40mJ) with a 30 min. recovery. In vitro kinase assay performed using AFT-2 as a substrate. ATF-2 phosphorylation detected using Phospho-ATF-2 (Thr71) Antibody #9221.
For in vitro kinase assays, we recommend a final ATP concentration of 200 µM.
Store at -20°C
Adenosine-5'-triphosphate (ATP) supplied as a 10 mM solution in doubly distilled water as a disodium salt.
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