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9852
Chaps Cell Extract Buffer (10X)
Buffers & Dyes
Buffer Kit

Chaps Cell Extract Buffer (10X) #9852

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To Purchase # 9852S
Product # Size Price
9852S
5 ml N/A

Product Includes Quantity
Chaps Cell Extract Buffer (10X) 5 ml
30X Reducing Agent (1.25 M DTT) 250 µl

Product Usage Information

Treat cells by adding fresh media containing regulator for desired time.

Aspirate media from cultures; wash cells three times with PBS; aspirate. Scrape cells into PBS and spin down to pellet.

Lyse cells by adding Chaps Cell Extract Buffer (1 volume of cell pellet). Resuspend cells in the buffer, freeze and thaw three times, centrifuge lysate at 14,000 rpm. Keep the supernatant and discard the pelleted cell debris.

Add SDS Sample Buffer and heat sample to 95–100°C for 5 minutes; cool on ice.

Microcentrifuge for 5 minutes.

Load 5 µL onto SDS-PAGE gel (10 cm x 10 cm).

Solutions and Reagents

CHAPS Cell Extract Buffer (5mL) 1X concentration: 50 mM Pipes/HCl (pH 6.5), 2 mM EDTA, 0.1% Chaps, 20 µg/ml Leupeptin, 10 µg/ml Pepstatin A,10 µg/ml Aprotinin. Add DTT to 5mM.

1.25 M (250X) DTT (0.25 mL).

Storage

Supplied as a 10X solution. Store at -20°C

Product Description

Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommended for the preparation of cytoplasmic cell lysates to be used with our caspase signaling pathway antibodies (see Companion Products).

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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