Phosphate Buffered Saline (PBS) solution with the detergent Tween® 20 for use as a wash buffer and diluent for ELISA. 1 X PBST contains 3.2 mM Na2HPO4, 0.5 mM KH2PO4, 1.3 mM KCl, 135 mM NaCl, 0.05% Tween® 20, pH 7.4.
Dilute Phosphate Buffered Saline with Tween® 20 (PBST - 20X) to a 1X solution using ddH2O. This product supplies enough 20X material to make 20 liters of 1X solution.
Supplied as a 20X solution. Store at room temperature.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Tween is a registered trademark of ICI Americas, Inc.
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