Dilute Tris Buffered Saline (TBS-10X) to a 1X solution using ddH2O. This product supplies enough 10X material to make 10 liters of 1X solution.
Store 10X buffer at room temperature. At 10X, this buffer is stable for 24 months. Use within 1 week once it has been diluted to 1X.
Tris buffered saline (TBS) solution for use as the blocking buffer diluent during fluorescent western blotting. Tween 20® detergent cannot be present in the blocking buffer because it can auto-fluoresce and increase non-specific background. After the blocking step, Tween 20® can be re-introduced to subsequent diluent buffers. Chemliluminescent western blotting does not require Tween 20® omission during blocking. Product is shipped and stored at room temperature.
1X Formulation: 137 mM Sodium Chloride, 20 mM Tris. Supplied at pH 7.6.
NOTE: This product does not contain Tween 20® detergent.