For the CUT&RUN assay, we recommend preparing 2 ml 1X Wash Buffer for each cell line and an additional 100 μl for each reaction or input sample. For example, to prepare 2.5 ml of 1X Wash Buffer, add 250 μl CUT&RUN 10X Wash Buffer, 25 μl 100X Spermidine #27287, and 12.5 μl Protease Inhibitor Cocktail (200X) #7012 to 2,212.5 μl nuclease-free water right before use. Equilibrate it to room temperature to minimize stress on the cells.
Store CUT&RUN 10X Wash Buffer at 4°C. This product is stable for at least 12 months.
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