For the CUT&RUN assay, we recommend adding 25 μl of Digitonin Solution per 1 ml buffer. Not all cell lines exhibit the same sensitivity to digitonin, so it may be necessary to adjust the final volume of digitonin in the buffer to get optimal permeabilization of your specific cell line. Cell permeabilization can be tested by combining an equal volume of cell suspension and 0.4% Trypan Blue Stain. Optimal cell permeabilization will result in staining of >90% of the cell population.
Digitonin Solution is supplied in nuclease-free water. Store at -20°C. This product is stable for at least 12 months.
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