Online offer: Dare to compare - Microsize Antibodies for £85 | Learn More >>
Human Brain-Derived Neurotrophic Factor (BDNF)

Human Brain-Derived Neurotrophic Factor (BDNF) #3897

Western Blotting Image 1

Western blot analysis of extracts from NIH/3T3 cells transfected with TrkB and treated with 50 ng/ml Brain-Derived Neurotrophic Factor (BDNF) for 5 minutes using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 (upper) and total TrkB (80G2) Rabbit mAb (lower).

Learn more about how we get our images

Recombinant human BDNF was expressed in E. coli and is supplied in a lyophilized form. A greater than 96% purity was determined by reverse phase-HPLC and SDS-PAGE.


> 96%

Working concentration of BDNF generally ranges from 50-100 ng/ml.


Recombinant human BDNF is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.

Neurotrophins are comprised of at least four family members including NGF, BDNF, NT-3 and NT-4 and all are known to influence growth, development, differentiation and survival of neurons (1). Proneurotrophis bind to p75NTR but not to the family of Trk receptor tyrosine kinases (Trk) and following maturation, BDNF binds and activates TrkB. Trk receptors in turn activate three major signaling pathways: (a) Ras-MAPK signaling, which promotes neuronal differentiation and neurite outgrowth, (b) PI3 Kinase-Akt signaling, which promotes survival and growth of neurons, and (c) PLC-γ1-PKC signaling, which promotes synaptic plasticity (2). BDNF is a major regulator of transmission and plasticity at adult synapses. Moreover, the precursor proBDNF and the mature protein mBDNF drive opposite effects on long-term potentiation and long-term depression (3). BDNF has also been implicated in body weight regulation and activity: heterozygous BDNF knockout mice are hyperphagic, obese, and hyperactive (4).

  1. Minichiello, L. and Klein, R. (1996) Genes Dev 10, 2849-58.
  2. Reichardt, L.F. (2006) Philos Trans R Soc Lond B Biol Sci 361, 1545-64.
  3. Martinowich, K. et al. (2007) Nat Neurosci 10, 1089-93.
  4. Kernie, S.G. et al. (2000) EMBO J 19, 1290-300.
Entrez-Gene Id
Swiss-Prot Acc.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Upstream / Downstream


Explore pathways related to this product.

Your Local Representative for Great Britain

Cell Signaling Technology

Hamilton House
Mabledon Place
London, WC1H 9BB

+44 1223 931164
0800 358 4693 (toll free)
+44 1223 931163

Need information for a different country? Please click here.

To get local purchase information on this product, click here.

Powered By OneLink