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79408
Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate)
Primary Antibodies

Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #79408

APPLICATIONS

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Akt (pan) (C67E7) Rabbit mAb #4691 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) (solid line) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control

#3900 (dashed line).

Learn more about how we get our images.

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for flow cytometry assays.

Storage:

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.

F(ab')2 fragments are prepared from goat antibodies that have been affinity purified and shown no reactivity against non-immunoglobulin rabbit serum proteins, serum proteins from bovine, human, or mouse and IgG from human or mouse based on immunoelectrophoresis (IEP) testing.

This product has been optimized for use as a secondary antibody in flow cytometry. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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To Purchase # 79408S

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Product # Size Price
79408S
250 µl N/A

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