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3323
Axin1 (C7B12) Rabbit mAb
Primary Antibodies
Monoclonal Antibody

Axin1 (C7B12) Rabbit mAb #3323

Citations (7)

We recommend the following alternatives

# Product Name Application Reactivity
  • WB
  • IP
  • IHC
H Mk
  • WB
  • IP
  • IHC
H M
No Current Image - Axin1 (C7B12) Rabbit mAb

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 110
Source/Isotype Rabbit 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Axin1 (C7B12) Rabbit mAb detects endogenous levels of total Axin1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein consisting of the N-terminal region of human Axin1.

Background

Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).

  1. Luo, W. and Lin, S.C. Neurosignals 13, 99-113.
  2. Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236.
  3. Zeng, X. et al. (2005) Nature 438, 873-877.
  4. Davidson, G. et al. (2005) Nature 438, 867-872.
  5. Zhang, Y. et al. (1999) J. Biol. Chem. 274, 35247-35254.
  6. Chia, I.V. and Costantini, F. (2005) Mol. Cell Biol. 25, 4371-4376.
  7. Jho, E.H. et al. (2002) Mol. Cell Biol. 22, 1172-1183.
  8. Lustig, B. et al. (2002) Mol. Cell Biol. 22, 1184-1193.
  9. Leung, J.Y. et al. (2002) J. Biol. Chem. 277, 21657-21665.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
To Purchase # 4315
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