Axin1 (C7B12) Rabbit mAb #3323

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Axin1 (C7B12) Rabbit mAb detects endogenous levels of total Axin1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein consisting of the N-terminal region of human Axin1.

Background

Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).

1. Luo, W. and Lin, S.C. Neurosignals 13, 99-113.
2. Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236.
3. Zeng, X. et al. (2005) Nature 438, 873-877.
4. Davidson, G. et al. (2005) Nature 438, 867-872.
5. Zhang, Y. et al. (1999) J. Biol. Chem. 274, 35247-35254.
6. Chia, I.V. and Costantini, F. (2005) Mol. Cell Biol. 25, 4371-4376.
7. Jho, E.H. et al. (2002) Mol. Cell Biol. 22, 1172-1183.
8. Lustig, B. et al. (2002) Mol. Cell Biol. 22, 1184-1193.
9. Leung, J.Y. et al. (2002) J. Biol. Chem. 277, 21657-21665.

Pathways & Proteins

Explore pathways + proteins related to this product.

Database Links

UniProt ID: O15169

Entrez-Gene Id: 8312

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