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9555
cdc25C Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

cdc25C Antibody Sampler Kit #9555

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cdc25C Antibody Sampler Kit: Image 1

Western blot analysis of extracts from HeLa, 293 and K-562 cells using cdc25C (5H9) Rabbit mAb #4688.

cdc25C Antibody Sampler Kit: Image 2

Western blot analysis of extracts from HT-29 cells, untreated, nocodazole-treated or λ phosphatase-treated, using Phospho-cdc25C (Ser216) Rabbit mAb #4901 (upper) and cdc25C (5H9) Rabbit mAb #4688 (lower).

cdc25C Antibody Sampler Kit: Image 3

Western blot analysis of extracts from HT-29 cells, untreated, nocodazole-treated or λ phosphatase-treated, using Phospho-cdc25C (Thr48) #9527 (upper) and cdc25C (5H9) Rabbit mAb #4688 (lower).

cdc25C Antibody Sampler Kit: Image 4

Western Blot analysis of HT29 cell extracts untreated, nocodazole-treated and lambda phosphotase-treated using Phospho-cdc25C (Thr48) (upper), and cdc25C (5H9) Rabbit mAb, #4688 (lower).

cdc25C Antibody Sampler Kit: Image 5

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

cdc25C Antibody Sampler Kit: Image 6

Western blot analysis of extracts from HeLa, 293 and K562 cells, using cdc25C (5H9) Rabbit mAb.

cdc25C Antibody Sampler Kit: Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

cdc25C Antibody Sampler Kit: Image 8

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

cdc25C Antibody Sampler Kit: Image 9

Western blot analysis of HT29 cell extracts untreated, nocodazole-treated, and λ phosphotase-treated using Phospho-cdc25C (Thr48), #9527 (upper), and cdc25C (5H9) Rabbit mAb (lower).

cdc25C Antibody Sampler Kit: Image 10

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

cdc25C Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb.

cdc25C Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human lymph node, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb in the presence of control peptide (left) or Phospho-cdc25C (Ser216) Blocking Peptide #1190 (right).

cdc25C Antibody Sampler Kit: Image 13

Western Blot analysis of HT29 cells, untreated, nocodazole-treated, and lambda phosphotase-treated, using Phospho-cdc25C (Ser216) antibody (upper), and cdc25C (5H9) Rabbit Mab, #4688, (lower).

cdc25C Antibody Sampler Kit: Image 14

Western blot analysis of GST-cdc25C, unphosphorylated or phosphorylated in vitro by Chk2, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb (upper) or cdc25C antibody #9522 (lower).

To Purchase # 9555T
Product # Size Price
9555T
1 Kit  (3 x 20 µl) N/A

Product Description

The cdc25C Antibody Sampler Kit provides an economical means to investigate the entry of eukaryotic cells into mitosis. The kit contains enough primary and secondary antibodies to perform two Western blots with each antibody.

Specificity / Sensitivity

Each antibody in the cdc25C Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length cdc25C protein or by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr48 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).

Full activation of cdc25C involves phosphorylation at more than 12 different sites by cdc2/cyclin B and Polo-like kinase, and the activity of Pin1, a peptidyl-prolyl isomerase (PPI) (6,7). Pin1 contains a WW domain that binds phospho-Ser/Thr-Pro sites and a catalytic PPI region that induces a cis/trans isomerization on phospho-Ser/Thr-Pro bonds (8). Thr48 and Thr67 of cdc25C interact directly with the WW domain of Pin1 when these sites are phosphorylated (9). Thr48 phosphorylation also mediates binding to CKS/p13SUC1 (10).

  1. Jessus, C. and Ozon, R. (1995) Prog Cell Cycle Res 1, 215-28.
  2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
  3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
  4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
  5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
  6. Izumi, T. and Maller, J.L. (1993) Mol Biol Cell 4, 1337-50.
  7. Stukenberg, P.T. and Kirschner, M.W. (2001) Mol Cell 7, 1071-83.
  8. Yaffe, M. B. et al. (1997) Science 278, 1957-1960.
  9. Lu, P. J. et al. (1999) Science 283, 1325-1328.
  10. Landrieu, I. et al. (2001) J Biol Chem 276, 1434-8.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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