Western blot analysis of extracts from various cell lines using DDX39A/UAP56 (E3J9O) Rabbit mAb.
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human DDX39A protein (hDDX39A-Myc/DDK; +) or untagged full-length human UAP56 protein (hUAP56; +), using DDX39A/UAP56 (E3J9O) Rabbit mAb.
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Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
DDX39A/UAP56 (E3J9O) Rabbit mAb recognizes endogenous levels of total DDX39A and UAP56 protein.
Human, Mouse, Rat, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro365 of human UAP56 protein.
The UAP56 gene is found in the central MHC region and encodes a member of the DEAD-box family of RNA helicases (1). Also known as DDX39B and BAT1, UAP56 functions as an ATP-dependent splicing factor and RNA helicase in the evolutionary conserved transcription/export (TREX) complex. The TREX complex is recruited to sites of active transcription, where it travels along the length of the gene with RNA polymerase II and exports resulting mRNAs to the cytoplasm (2-8).
DDX39, also known as DDX39A, is a DEAD-box helicase highly homologous to UAP56 and is upregulated in lung squamous cell cancers (9,10). DDX39 has been shown to bind to TRF2 to regulate telomere protection (11). Furthermore, DDX39 is overexpressed in several cancer types, which is associated with poor prognosis (12-15). Both UAP56 and DDX39 are hijacked by various viral replication machineries to enable viral reproduction and mRNA export (16-18). UAP56 and DDX39 have also been implicated in promoting the AR-V7 splice variant in advanced prostate cancers (19).
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