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4759
ER Protein Folding Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

ER Protein Folding Antibody Sampler Kit #4759

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ER Protein Folding Antibody Sampler Kit: Image 1

Flow cytometric analysis of A204 cells using BiP (C50B12) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

ER Protein Folding Antibody Sampler Kit: Image 2

Western blot analysis of extracts from various cell lines, using Ero1-Lα Antibody.

ER Protein Folding Antibody Sampler Kit: Image 3

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ERp44 (D17A6) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

ER Protein Folding Antibody Sampler Kit: Image 4

Western blot analysis of extracts from various cell types using ERp57 (G117) Antibody.

ER Protein Folding Antibody Sampler Kit: Image 5

Flow cytometric analysis of Hep G2 cells using ERp72 (D70D12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

ER Protein Folding Antibody Sampler Kit: Image 6

Western blot analysis of extracts from various cell types using Grp94 Antibody.

ER Protein Folding Antibody Sampler Kit: Image 7

Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

ER Protein Folding Antibody Sampler Kit: Image 8

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

ER Protein Folding Antibody Sampler Kit: Image 9

Immunohistochemical analysis of paraffin-embedded human glioblastoma using BiP (C50B12) Rabbit mAb.

ER Protein Folding Antibody Sampler Kit: Image 10

Western blot analysis of extracts from various cell types using ERp44 (D17A6) XP® Rabbit mAb.

ER Protein Folding Antibody Sampler Kit: Image 11

Confocal immunofluorescent analysis of PANC-1 cells using ERp72 (D70D12) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

ER Protein Folding Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI (C81H6) Rabbit mAb.

ER Protein Folding Antibody Sampler Kit: Image 13

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using BiP (C50B12) Rabbit mAb.

ER Protein Folding Antibody Sampler Kit: Image 14

Western blot analysis of extracts from various cell lines using ERp72 (D70D12) XP® Rabbit mAb.

ER Protein Folding Antibody Sampler Kit: Image 15

Immunohistochemical analysis of paraffin-embedded human lymphoma using PDI (C81H6) Rabbit mAb.

ER Protein Folding Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using BiP (C50B12) Rabbit mAb.

ER Protein Folding Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded mouse spleen using PDI (C81H6) Rabbit mAb.

ER Protein Folding Antibody Sampler Kit: Image 18

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using BiP (C50B12) Rabbit mAb in the presence of control peptide (left) or BiP Blocking Peptide #1084 (right).

ER Protein Folding Antibody Sampler Kit: Image 19

Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb.

ER Protein Folding Antibody Sampler Kit: Image 20

Western blot analysis of extracts from various cell lines using BiP (C50B12) Rabbit mAb.

To Purchase # 4759T
Product # Size Price
4759T
1 Kit  (7 x 20 µl) N/A

Product Description

The ER Protein Folding Antibody Sampler Kit contains reagents to investigate the initiation of translation within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.

Specificity / Sensitivity

Each antibody in the ER Protein Folding Antibody Sampler Kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Gly584 of human BiP, the sequence of human ERp44, the residues surrounding Met279 of human ERp72 protein and the sequence of human PD1. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequences around Gly117 of human ERp57, Met622 of human Grp94 and Leu218 of human Ero1-Lα. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

After their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation on folding proteins (3). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress proteins 72, 57 and 44 (ERp72, ERp57 and ERp44), constitute the PDI family (4,5,6). The ER also contains a pool of molecular chaperones, including Grp94, to help proteins fold properly. Grp94 is a glucose-regulated protein (7) with sequence homology to Hsp90 (8). BiP is another chaperone whose synthesis is increased when protein folding is disturbed. BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (9).

  1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-148.
  2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep. 6, 28-32.
  3. Tu, B.P. and Weissman, J.S. (2004) J. Cell Biol. 164, 341-346.
  4. Mazzarella, RA et al. (1990) J Biol Chem 265(2), 1094-101.
  5. Satoh, M et al. (2005) Cell Stress Chaperones 10(4), 278-84
  6. Jessop, C.E. et al. (2007) EMBO J 26, 28-40.
  7. Lee, A.S. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4922-4925.
  8. Sorger, P.K. and Pelham, H.R. (1987) J. Mol. Biol. 194, 341-344.
  9. Kohno, K. et al. (1993) Mol Cell Biol 13, 877-90.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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