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48267
Fragile X/FMRP Signaling Pathway Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit #48267

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Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 1

Confocal immunofluorescent analysis of adult mouse cerebellum tissue using mGluR1 (D5H10) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 2

Confocal immunofluorescent analysis of mouse hippocampus (left) and striatum (right) using mGluR5 (D6E7B) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 3

Confocal immunofluorescent analysis of C2C12 cells, untreated (left) or treated with MG132 (10 μg/mL, 3 hrs; right), using FMRP (D14F4) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 4

Confocal immunofluorescent analysis of C2C12 cells, untreated (left) or MG-132 treated (10 μg/mL, 3 hr; right), using FXR1 (D10A2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 5

Confocal immunofluorescent analysis of C2C12 cells, untreated (left) or MG132-treated (10 μg/mL, 3 hr, right) using FXR2 (D85D6) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 6

Western blot analysis of CAKI-1 and SU-DHL-4 cells, and human brain extracts, using CYFIP1 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 7

Western blot analysis of extracts from C6 cells, untreated or forskolin-treated (10 µM for 60 minutes), using Phospho-eEF2 (Thr56) Antibody (upper) or eEF2 Antibody #2332 (lower).

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 8

Confocal immunofluorescent analysis of HeLa cells using eEF2 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 10

Immunohistochemical analysis of paraffin-embedded mouse cerebellum using mGlur1 (D5H10) Rabbit mAb.

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 11

Immunoprecipitation of mGluR5 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is mGluR5 (D6E7B) Rabbit mAb. Western blot analysis was performed using mGluR5 (D6E7B) Rabbit mAb.

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 12

Western blot analysis of extracts from various cell lines using FMRP (D14F4) Rabbit mAb.

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 13

Western blot analysis of extracts from various cell lines using FXR1 (D10A2) XP® Rabbit mAb.

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 14

Western blot analysis of extracts from various cell lines using FXR2 (D85D6) Rabbit mAb.

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 15

Immunohistochemical analysis of paraffin-embedded rat retina using mGlur1 (D5H10) Rabbit mAb.

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 16

Western blot analysis of extracts from the indicated tissues using mGluR5 (D6E7B) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 17

Western blot analysis of extracts from human and mouse skeletal muscle tissues using FXR1 (D10A2) XP® Rabbit mAb.

Fragile X/FMRP Signaling Pathway Antibody Sampler Kit: Image 18

Western blot analysis of extracts from neonatal mouse brain and rat brain using mGluR1 (D5H10) Rabbit mAb.

To Purchase # 48267T
Product # Size Price
48267T
1 Kit  (8 x 20 µl) N/A

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
mGluR1 (D5H10) Rabbit mAb 12551 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R 145, >300 Rabbit IgG
mGluR5 (D6E7B) Rabbit mAb 55920 20 µl
  • WB
  • IP
  • IF
H M R 150, 300 Rabbit IgG
FMRP (D14F4) Rabbit mAb 7104 20 µl
  • WB
  • IF
H M R Mk 80 Rabbit IgG
FXR1 (D10A2) XP® Rabbit mAb 12295 20 µl
  • WB
  • IF
H M R Mk 78-80, 82-84 Rabbit IgG
FXR2 (D85D6) Rabbit mAb 7098 20 µl
  • WB
  • IF
H M R Mk 95 Rabbit IgG
CYFIP1 Antibody 44353 20 µl
  • WB
H M 145 Rabbit 
Phospho-eEF2 (Thr56) Antibody 2331 20 µl
  • WB
H M R Hm Mk C 95 Rabbit 
eEF2 Antibody 2332 20 µl
  • WB
  • IF
H M R Mk Dm 95 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Fragile X/FMRP Signaling Pathway Antibody Sampler Kit provides an economical means of detecting signaling components of the Fragile X/FMRP signaling pathway. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Fragile X/FMRP Signaling Pathway Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-eEF2 (Thr56) Antibody detects endogenous levels of eEF2 only when phosphorylated at Thr56. It does not recognize eEF2 phosphorylated at other sites.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Gly552 of human FMRP protein, Leu1105 of human mGluR1 protein, Gly574 of human FXR1 protein, and Gly477 of human FXR2 protein. mGluR5 (D6E7B) Rabbit mAb is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human mGluR5 protein. eEF2 Antibody and CYFIP1 Antibody are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino-terminus of human eEF2 and residues surrounding Pro527 of human CYFIP1 protein, respectively. Phospho-eEF2 (Thr56) Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr56 of human eEF2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Fragile X syndrome, a frequent cause of inherited mental retardation, often results from expansion of the CGG trinucleotide repeat in the gene that encodes the fragile X mental retardation protein (FMRP, [1]). FMRP (also known as FMR1) and its two autosomal homologs (FXR1 and FXR2) all bind RNA and play a role in the pathogenesis of fragile X syndrome (1-3). Each of these related proteins can associate with one another as well as form homodimers and complexes with other RNA-binding proteins like cytoplasmic FMRP interacting protein 1 (CYFIP1, [3,4]). FMRP, FXR1, FXR2, and CYFIP1 have been implicated in the translational regulation of mRNAs (5,6). Importantly, this complex of proteins may be dynamically regulated to drive protein synthesis-dependent forms of synaptic plasticity in response to specific activity. That is, activation of metabotropic glutamate receptors, including mGluR1 and mGlur5, can regulate FMRP-dependent forms of translation via post-translational modification of eukaryotic elongation factor 2 (eEF2) to locally control dynamic translation of important synaptic proteins, which, subsequently, alter synaptic function (7-9).   

  1. Verkerk, A.J. et al. (1991) Cell 65, 905-14.
  2. Siomi, M.C. et al. (1995) EMBO J 14, 2401-8.
  3. Zhang, Y. et al. (1995) EMBO J 14, 5358-66.
  4. Abekhoukh, S. et al. (2017) Dis Model Mech 10, 463-74.
  5. Linder, B. et al. (2008) Hum Mol Genet 17, 3236-46.
  6. De Rubeis, S. et al. (2013) Neuron 79, 1169-82.
  7. Park, S. et al. (2008) Neuron 59, 70-83.
  8. Barnes, S.A. et al. (2015) J Neurosci 35, 15073-81.
  9. Paul, A. et al. (2019) Front Mol Neurosci 12, 97.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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