Western blot analysis of extracts from mouse brain, rat brain, and human cerebellum using GRAF1 Antibody.
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Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
GRAF1 Antibody recognizes endogenous levels of total GRAF1 protein.
Human, Mouse, Rat
Monkey, Bovine, Horse
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys261 of human GRAF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
GTPase Regulator Associated with Focal Adhesion Kinase-1 (GRAF1), is a GTPase-activating protein for the small G proteins RhoA and Cdc42 (1). It is composed of an N-terminal BAR domain, a PH domain, a RhoGAP domain, a proline-rich domain, and a C-terminal SH3 domain. GRAF1 contributes to the clathrin-independent carriers/GPI-enriched early endosomal compartments (CLIC/GEEC) pathway, and was the first specific protein component of this endocytic pathway to be discovered (2). GRAF1 was identified as an important protein necessary for adeno-associated virus 2 infection (3). In addition, research studies have linked GRAF1 to mental retardation (4), skeletal muscle differentiation (5), and myeloid leukemia (6,7).
Explore pathways + proteins related to this product.
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