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33893
Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free) #33893

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Filter:
  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from control HeLa cells (lane 1) or β-Catenin knockout HeLa cells (lane 2) using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The absence of signal in the β-Catenin knockout HeLa cells confirms the specificity of the antibody for β-Catenin. Data were generated using the standard formulation of this product.
Western Blotting Image 2: Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from 293T cells, untreated (-) or treated (+) with MG132 (10 μM, 4 hr at 37ºC), using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (left), Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561 (center), or β-Catenin (6B3) Rabbit mAb #9582 (right). Note that Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb fails to detect poly-ubiquitinated β-catenin in MG132-treated cells, indicating its specificity for stabilized protein. Data were generated using the standard formulation of this product.
Western Blotting Image 3: Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from 293T cells, untreated (-) or treated (+) with LiCl (20 mM, 20 hr at 37ºC), using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (left) and β-Catenin Antibody #9562 (right). Equal protein loading was confirmed using β-Tubulin (9F3) Rabbit mAb #2128. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin embedded human breast carcinoma using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma (left) and adjacent normal intestinal epithelium (right) using Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb. Note the nuclear accumulation of active beta-Catenin in the adenoma cells. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin embedded mouse colon using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb in the presence of phospho-β-catenin (Ser33/37/Thr41) peptide (left) or non-phospho-β-catenin (Ser33/37/Thr41) peptide (right). Note the absence of staining only in the presence of the non-phospho-β-catenin (Ser33/37/Thr41) peptide, demonstrating non-phospho specificity. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin embedded cell pellets, HeLa (left) or NCI-H28 (right), using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HCT-15 (left, center; positive) or NCI-H28 (right; negative) cells using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (green) in the presence of Phospho-Catenin-β (Ser33/Ser37/Thr41) peptide (left) or Non-phospho-Catenin-β (Ser33/Ser37/Thr41) peptide (center). Red = Propidium Iodide (PI)/RNase Staining Solution #4087. Data were generated using the standard formulation of this product.
To Purchase # 33893
Cat. # Size Qty. Price
33893SF
100 µg N/A

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 92
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #8814. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #8814.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (BSA and Azide Free) recognizes endogenous β-catenin protein when residues Ser33, Ser37, and Thr41 are not phosphorylated. It does not detect β-catenin protein if tri-phosphorylated at Ser33/Ser37/Thr41. This antibody may also detect β-catenin protein when singly phosphorylated at Ser33. This specificity data was derived from competition ELISA and dot blot analysis using synthetic peptides.

Species Reactivity:

Human, Mouse, Rat, Monkey

Species predicted to react based on 100% sequence homology:

Chicken, D. melanogaster, Xenopus, Bovine, Dog

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human β-catenin protein.

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines (8).
Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb is designed to specifically recognize the stabilized form of β-catenin, i.e., protein that has not been phosphorylated by GSK-3, and thus is functionally active in cell-cell adhesion and/or the canonical Wnt signaling pathway.
  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-3305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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