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64097
Plasma Membrane Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Plasma Membrane Marker Antibody Sampler Kit #64097

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Simple Western™ analysis of lysates (0.1 mg/mL) from MCF-7 cells using E-Cadherin (24E10) Rabbit mAb #3195. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Confocal immunofluorescent analysis of fixed frozen mouse pancreas labeled with Caveolin-1 (D46G3) XP® Rabbit mAb (left, red), and colabeled with DyLight 488 Phalloidin #12935 (right, green) and DAPI #4083 (right, blue).
Confocal immunofluorescent analysis of fixed frozen mouse kidney labeled with Caveolin-1 (D46G3) XP® Rabbit mAb (left, red), and colabeled with DyLight 488 Phalloidin #12935 (right, green) and DAPI #4083 (right, blue).
Confocal immunofluorescent analysis of fixed frozen mouse skeletal muscle labeled with Caveolin-1 (D46G3) XP® Rabbit mAb (left, red), and colabeled with DyLight 488 Phalloidin #12935 (right, green) and DAPI #4083 (right, blue).
Western blot analysis of extracts from A172 and MCF7 cells using N-Cadherin (D4R1H) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 2 min; +), and HeLa cells, untreated (-) or pervanadate-treated (1 mM, 15 min; +), using Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb (upper) and Na,K-ATPase Antibody #3010 (lower).
Western blot analysis of extracts from SK-MEL-28, SNB19 and A-204 cells using Na,K-ATPase α1 (D4Y7E) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Western blot analysis of extracts from various cell lines, using E-Cadherin (24E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using E-Cadherin (24E10) Rabbit mAb performed on the Leica BOND Rx.
Western blot analysis of extracts from various cell types using Caveolin-1 (D46G3) XP® Rabbit mAb.
Western blot analysis of extracts from various cell lines, using Pan-Cadherin (28E12) Rabbit mAb.
Western blot analysis of extracts from various cell lines using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from Hep G2 and MDA-MB-231 cells using ENPP1 (D37B7) Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin Lymphoma using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded SK-MEL-28 (left) and A-204 (right) cell pellets using Na,K-ATPase α1 (D4Y7E) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human papillary thyroid carcinoma using E-Cadherin (24E10) Rabbit mAb performed on the Leica BOND Rx.
Western blot analysis of extracts from control HeLa cells (lane 1) or caveolin-1 knockout HeLa cells (lane 2) using Caveolin-1 (D46G3) XP® Rabbit mAb #3267 (upper), or β-actin (D6A8) Rabbit mAb #8457 (lower). The absence of signal in the caveolin-1 knockout HeLa cells confirms the specificity of the antibody to caveolin-1.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded human appendix using Na,K-ATPase α1 (D4Y7E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using E-Cadherin (24E10) Rabbit mAb.
Immunoprecipitation of caveolin-1 from HeLa cells using Caveolin-1 (D46G3) XP® Rabbit mAb followed by western blot using the same antibody. Lane 1 is 5% input.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using N-Cadherin (D4R1H) XP® Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Na,K-ATPase α1 (D4Y7E) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma in lymph node, using E-Cadherin (24E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Caveolin-1 (D46G3) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon using N-Cadherin (D4R1H) XP® Rabbit mAb. Note staining of myenteric plexus.
Immunohistochemical analysis of paraffin-embedded mouse prostate using E-Cadherin (24E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lymphoma using Caveolin-1 (D46G3) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using N-Cadherin (D4R1H) XP® Rabbit mAb.
Confocal immunofluorescent analysis of SK-MEL-28 (left), SNB19 (middle) or A-204 (right) cells using Na,K-ATPase α1 (D4Y7E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded mouse pancreas using E-Cadherin (24E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse lung using Caveolin-1 (D46G3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Confocal immunofluorescent analysis of HeLa (left, high expressing) or SH-SY5Y (right, low expressing) cells using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded A172 (positive, left) and MCF7 (negative, right) cell pellets using N-Cadherin (D4R1H) XP® Rabbit mAb.
Confocal immunofluorescent images of MCF7 cells using E-Cadherin (24E10) Rabbit mAb (green, left) compared to an isotype control (right). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded mouse small intestine using E-Cadherin (24E10) Rabbit mAb.
Confocal immunofluorescent analysis of C2C12 cells using Caveolin-1 (D46G3) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Flow cytometric analysis of SH-SY5Y cells (blue) and HeLa cells (green) using 4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Confocal immunofluorescent analysis of A172 (positive, left) and MCF7 (negative, right) cells using N-Cadherin (D4R1H) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded mouse lung using E-Cadherin (24E10) Rabbit mAb.
Flow cytometric analysis of HeLa cells using Caveolin-1 (D46G3) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded mouse stomach using E-Cadherin (24E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using E-Cadherin (24E10) Rabbit mAb in the presence of control peptide (left) or E-Cadherin Blocking Peptide #1050 (right).
Flow cytometric analysis of Jurkat cells (blue, negative) and MCF7 cells (green, positive) using E-Cadherin (24E10) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
To Purchase # 64097
Cat. # Size Qty. Price
64097T
1 Kit  (8 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb 13566 20 µl
  • WB
H M 100 Rabbit IgG
Pan-Cadherin (28E12) Rabbit mAb 4073 20 µl
  • WB
H M R 130-150 Rabbit IgG
E-Cadherin (24E10) Rabbit mAb 3195 20 µl
  • WB
  • IHC
  • IF
  • F
H M 135 Rabbit IgG
4F2hc/SLC3A2 (D3F9D) XP® Rabbit mAb 47213 20 µl
  • WB
  • IHC
  • IF
  • F
H 75-120 Rabbit IgG
Caveolin-1 (D46G3) XP® Rabbit mAb 3267 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R Hm Mk B Dg 21, 24 Rabbit IgG
N-Cadherin (D4R1H) XP® Rabbit mAb 13116 20 µl
  • WB
  • IP
  • IHC
  • IF
H M 140 Rabbit IgG
ENPP1 (D37B7) Rabbit mAb 5342 20 µl
  • WB
H 140 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Rab Goat 
Na,K-ATPase α1 (D4Y7E) Rabbit mAb 23565 20 µl
  • WB
  • IHC
  • IF
H 100 Rabbit IgG

Product Description

The Plasma Membrane Marker Antibody Sampler Kit provides an economical means of detecting plasma membrane markers in various cell types. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Plasma Membrane Marker Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-Na,K-ATPase α1 (Tyr10) (E1Y9C) Rabbit mAb recognizes endogenous levels of Na,K-ATPase α1 protein only when phosphorylated at Tyr10, and cross-reacts with an induced 75-80 kDa doublet of unknown origin. Pan-Cadherin (28E12) Rabbit mAb detects endogenous levels of total cadherin proteins. 4F2hc/CD98 (D3F9D) XP® Rabbit mAb recognizes endogenous levels of total 4F2hc/CD98 protein, and is predicted to detect multiple isoforms of 4F2hc/CD98. 

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro12 of human Na,K-ATPase α1, Pro780 of human E-cadherin, Arg526 of human N-cadherin, Val509 of human 4F2hc/CD98, Glu20 of human caveolin-1, Leu520 of human ENPP1, a conserved region of human N-, R-, E- and P-cadherin proteins, or a synthetic phosphopeptide corresponding to residues surrounding Tyr10 of human Na,K-ATPase α1 protein.

Background

The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. Phosphorylation of Na,K-ATPase at Tyr10 has been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters (1). Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells (2). 4F2hc is a transmembrane protein that belongs to the solute carrier family. 4F2hc forms heterodimeric complexes with various amino acid transporters, such as LAT1 and LAT2, and regulates uptake of amino acids (3). The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions (4). Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. ENPP1 plays important roles in bone mineralization and soft tissue calcification (5).

Pathways

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Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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U.S. Patent No. 5,675,063.
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