Confocal immunofluorescent analysis of the hypothalamic region of normal mouse brain (left) and a magnified image (right) using Vasopressin (D8T3K) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Recommended Fluorochrome-conjugated Anti-Rabbit secondary antibodies:
NOTE: When using any primary or fluorochrome-conjugated secondary antibody for the first time, titrate the antibody to determine which dilution allows for the strongest specific signal with the least background for your sample.
Cover sections with 4% formaldehyde dilute in 1X PBS.
NOTE: Formaldehyde is toxic, use only in fume hood.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised July 2016
Protocol Id: 151
Vasopressin (D8T3K) Rabbit mAb recognizes endogenous levels of total Vasopressin protein. Presumed non-specific labeling of retinal plexiform layers has been observed by immunofluorescence.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro155 of human Vasopressin protein.
Vasopressin is a neuroendocrine peptide that is released to the circulation by magnocellular neurons whose cell bodies are mainly found in the paraventricular and the supraoptic nuclei of the hypothalamus. It was first isolated from pituitary gland extracts and synthesized in 1951 (1). Vasopressin acts by activating G protein-coupled, V1a, V1b (also known as V3) and V2 receptors and plays a fundamental role in the maintenance of water homeostasis. One of its main functions is body water retention (2), hence its alternative name antidiuretic hormone or ADH. Vasopressin also leads to increased arterial blood pressure by raising peripheral vascular resistance (3). Vasopressin is also involved in other physiological processes such as acute heart failure (4), pain (5), and metabolic syndrome (6).
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