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83986
Human ACE2 (18-652) Recombinant Protein (mFc-Tag)
Recombinant Proteins
Protein Control

Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986

Citations (0)
Binding response kinetics between Human ACE2 (18-652) Recombinant Protein (mFc-Tag) (immobilized) and SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 (in solution, at indicated concentrations). The vertical red line (180 sec) indicates addition of PBS to induce dissociation. Binding was detected with an anti-mouse Fc biosensor. Values on y-axis indicate binding response signals recorded for 6 different concentrations of SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 (31.2, 62.5, 125, 250, 500 and 1000 nM).
Binding response kinetics between Human ACE2 (18-652) Recombinant Protein (mFc-Tag) (immobilized) and SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag) #48801 (in solution, at indicated concentrations). The vertical red line (180 sec) indicates addition of PBS to induce dissociation. Binding was detected with an anti-mouse Fc biosensor. Values on y-axis indicate binding response signals recorded for 5 different concentrations of SARS-CoV-2 Spike RBD (318-541) Recombinant Protein (8xHis-Tag) #48801 (62.5, 125, 250, 500 and 1000 nM).
The purity of Human ACE2 (18-652) Recombinant Protein (mFc-Tag) was determined by densitometry after SDS-PAGE of 2 μg of protein, followed by staining with Coomassie Blue. Purity values were determined from the DTT-reduced samples (+).

Supporting Data

MW (kDa) 120 (reduced); 140, 250 (non-reduced)

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Formulation

Expression Host: Human (HEK293 cells)
Supplied in a PBS solution (pH 7.2).

Storage

Stable at -80°C for 3 years after receipt. Avoid repeated freeze-thaw cycles.

Product Description

Human ACE2 (18-652) Recombinant Protein (mFc-Tag) is derived from a recombinant expression construct corresponding to the extracellular domain of human ACE2 protein, including the sequence regions that function as receptors for SARS and SARS-CoV-2 coronaviruses. The expressed protein contains a murine Fc-Tag at its carboxy terminus.
MW (kDa) 120 (reduced); 140, 250 (non-reduced)
Purity 80%, determined by SDS-PAGE.

Background

ACE2 is a carboxypeptidase that catalyses the conversion of angiotensin I to angiotensin 1-9, or of angiotensin II to the vasodilator angiotensin 1-7 (1). ACE2 is a critical component in the renin-angiotensin system (RAS). ACE2 is predominantly expressed in vascular endothelial cells of the heart and kidney and Leydig and Sertoli cells of the testis (2,3). The unique expression pattern of ACE2 determines its essential role in the regulation of cardiovascular and kidney functions, as well as fertility. ACE2 protein is localized mainly in the extracellular space with its carboxy terminal end attached to the membrane via its transmembrane domain. Active ACE2 enzyme is secreted by cleavage at the amino terminus. Research studies have shown that ACE2 expression is elevated in human failing heart (4). ACE2 has also been identified as the receptor for SARS and SARS-CoV-2 coronaviruses (5-7).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 83986
Cat. # Size Qty. Price
83986S
20 µg