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4409
Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
Secondary Antibodies

Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4409

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  1. IF

Confocal immunofluorescent analysis of HeLa cells using S6 Ribosomal Protein (54D2) Mouse mAb #2317 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

High content analysis of C2C12 cells exposed to varying concentrations of LY294002 #9901 for 2 hours. With increasing concentrations of LY294002, a significant decrease (~20 fold) in phospho-S6 Ribosomal protein (Ser235/236) signal as compared to the untreated control was observed. When using phospho-S6 Ribosomal protein as a measurement, the IC50 of this compound was 2.3 μM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate).

To Purchase # 4409S
Product # Size Price
4409S
250 µl N/A

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa)
Isotype Goat 

Product Description

Anti-Mouse IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.

Product Usage Information

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500 - 1:2000 should yield acceptable results for immunofluorescent assays.

Storage:

Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.

Background

This product has been optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Acumen is a registered trademarks of TTP Labtech.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
DRAQ5 is a registered trademark of Biostatus Limited.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation, for research use only for immunocytometry, immunohistochemistry, high content screening (HCS) analysis, or flow cytometry applications.
The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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