Online offer: Dare to compare - Microsize Antibodies for £85 | Learn More >>
SignalSilence® ERCC1 siRNA I

SignalSilence® ERCC1 siRNA I #8896

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® ERCC1 siRNA I (+), using ERCC1 (D61F5) Rabbit mAb #5437 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The ERCC1 (D61F5) Rabbit mAb confirms silencing of ERCC1 expression while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

Learn more about how we get our images

CST recommends transfection with 100 nM SignalSilence® ERCC1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

SignalSilence® ERCC1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ERCC1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression by western analysis.

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with ERCC4/XPF, which acts as the 5’ endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC) (5,6).

  1. Shuck, S.C. et al. (2008) Cell Res 18, 64-72.
  2. McDaniel, L.D. and Schultz, R.A. (2008) Adv Exp Med Biol 637, 65-82.
  3. Niedernhofer, L.J. et al. (2004) Mol Cell Biol 24, 5776-87.
  4. Ahmad, A. et al. (2008) Mol Cell Biol 28, 5082-92.
  5. Zheng, Z. et al. (2007) N Engl J Med 356, 800-8.
  6. Gossage, L. and Madhusudan, S. (2007) Cancer Treat Rev 33, 565-77.
Entrez-Gene Id
Swiss-Prot Acc.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

Upstream / Downstream


Explore pathways related to this product.

Important Ordering Details

Custom Ordering Details: Product is assembled upon order. Please allow up to three business days for your product to be processed.

To Purchase # 8896S

View sizes
Product # Size Price
300 µl  (3 nmol) N/A

Your Local Representative for Great Britain

Cell Signaling Technology

Hamilton House
Mabledon Place
London, WC1H 9BB

+44 1223 931164
0800 358 4693 (toll free)
+44 1223 931163

Need information for a different country? Please click here.

To get local purchase information on this product, click here.

Powered By OneLink