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SignalSilence® ERK5 siRNA I

SignalSilence® ERK5 siRNA I #7301

Western Blotting

Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® ERK5 siRNA I (+), using Erk5 (D23E9) Rabbit mAb #3552 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Erk5 (D23E9) Rabbit mAb confirms silencing of ERK5 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

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CST recommends transfection with 100 nM SignalSilence® ERK5 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.


SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

SignalSilence® ERK5 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ERK5 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Erk5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival (1-4). Like other MAPK family members, Erk5 contains a canonical activation loop TEY motif (Thr218/Tyr220) that is specifically phosphorylated by MAP2K5 (MEK5) in a growth-factor-dependent, Ras-independent mechanism (5-7). For example, EGF stimulation promotes Erk5 phosphorylation that induces its translocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (5,6). Erk5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoietic progenitor cells where it promotes survival and proliferation (7). In neuronal cells, Erk5 is required for NGF-induced neurite outgrowth, neuronal homeostasis, and survival (8,9). Erk5 is thought to play a role in blood vessel integrity via maintenance of endothelial cell migration and barrier function (10-12). Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting Erk5 plays a critical role in vascular development and homeostasis (1,2).

  1. Zhou, G. et al. (1995) J Biol Chem 270, 12665-9.
  2. Hayashi, M. and Lee, J.D. (2004) J Mol Med 82, 800-8.
  3. Wang, X. and Tournier, C. (2006) Cell Signal 18, 753-60.
  4. Nishimoto, S. and Nishida, E. (2006) EMBO Rep 7, 782-6.
  5. Kato, Y. et al. (1998) Nature 395, 713-6.
  6. Kato, Y. et al. (1997) EMBO J 16, 7054-66.
  7. Dong, F. et al. (2001) J Biol Chem 276, 10811-6.
  8. Obara, Y. et al. (2009) J Biol Chem 284, 23564-73.
  9. Finegan, K.G. et al. (2009) Cell Death Differ 16, 674-83.
  10. Spiering, D. et al. (2009) J Biol Chem 284, 24972-80.
  11. Sawhney, R.S. et al. (2009) J Cell Physiol 219, 152-61.
  12. Zhao, Z. et al. (2009) Mol Cell Biochem 322, 171-8.
Entrez-Gene Id
Swiss-Prot Acc.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

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Important Ordering Details

Custom Ordering Details: Product is assembled upon order to ensure maximum activity. United States: Please allow up to two weeks for your order to be processed and shipped. Outside of the United States: Please allow up to three weeks, depending on the country, for your order to be processed and shipped. When ordering five or more kits, please contact us for processing time and pricing at

To Purchase # 7301S

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Product # Size Price
300 µl  (3 nmol) N/A

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