Standard curves generated with bovine serum albumin (BSA) ranging from 125 to 2,000 μg/ml using Cell Lysis Buffer #9803, RIPA Buffer #9806, and PathScan® Sandwich ELISA Lysis Buffer #7018.
|BCA Protein Assay Reagent A||250 ml|
|BCA Protein Assay Reagent B||25 ml|
|BCA Protein Assay Albumin Standard||10 units|
|Compatibility Reagent||48 ea|
|Reconstitution Buffer||15 ml|
Store at room temperature.
The BCA Protein Assay Kit can be used to measure the protein concentration of lysates or homogenates, in microplate format, prepared with the following buffers: Cell Lysis Buffer (10X) #9803, RIPA Buffer (10X) #9806, PathScan® Sandwich ELISA Lysis Buffer (1X) #7018. The dynamic range for this assay is 0.125 - 2 mg/mL. It is recommended that the BCA Compatibility Reagent be used to decrease interference from reducing agents, chelators, detergents, and other common ingredients found in most lysis buffers. Please see the attached protocol for additional details.
Bicinchoninic Acid (BCA) is capable of forming an intense purple complex with cuprous ion, Cu1+, in an alkaline environment. Cu1h+ is produced from the reaction of protein with alkaline Cu2+. The resulting reaction and color produced is the basis for a common protein quantification method capable of measuring protein concentration over a wide range. Increasing protein concentrations produce proportionally deeper colors. The BCA protein assay demonstrates higher tolerances towards common interfering substances, such as nonionic detergents and buffer salts, than the Lowry technique (1).
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