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PTMScan® Motif Antibody Kits

The PTMScan® Motif Antibody Kits are a product line that enables the use of PTMScan Technology, a proprietary technology developed at Cell Signaling Technology (CST). The kits provide a selection of our proprietary Motif Antibodies ready for use in studies that employ PTMScan Technology. These antibodies are identical to those utilized by scientists at Cell Signaling Technology in PTMScan Discovery Services. For our customers with in-house expertise in mass spectrometry proteomics, these kits provide a cost-effective means of accessing any of the CST™ Motif IAP Beads and a Limited Use License to perform PTMScan Technology. Reagents for 10 assays as well as detailed protocols are provided in each kit.

Why consider antibody enrichment for studying phosphorylation?

  • Focused discovery of phosphosites based on amino acid (S/T/Y) or motif of interest
  • Increased coverage of lower occurring phosphorylation events compared to IMAC
  • View data showing complementarity between antibody enrichment and IMAC

PTMScan® Motif Antibody Kits

No. PTMScan® Kit Description Recommended Protease Treatment Modification Type LCMS Data & Output Results
No. PTMScan® Kit Description Recommended Protease Treatment Modification Type LCMS Data & Output Results
59322 PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit Trypsin

WALP		 Ubiquitin

SUMOylation		 Mouse Liver
XLS  |  RAW
HEK293 Cells
XLS  |  RAW
13416 PTMScan® Acetyl-Lysine Motif [Ac-K] Kit Trypsin Acetylation Mouse Liver
XLS  |  RAW 1 2
14499 PTMScan® Pilot Acetyl-Lysine Motif [Ac-K] Kit Trypsin Acetylation Mouse Liver
XLS  |  RAW 1 2
5561 PTMScan® Phospho-Akt Substrate Motif mAb 1 (RXXS*/T*) Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW 1 2
5563 PTMScan® Phospho-Akt Substrate Motif mAb 2 (RXRXXS*/T*) Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW 1 2
5564 PTMScan® Phospho-AMPK Substrate Motif (LXRXXS*/T*) Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW 1 2
12267 PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW 1 2
12235 PTMScan® Mono-Methyl Arginine Motif [mme-RG] Kit Trypsin* Methylation Mouse Liver
XLS  |  RAW 1 2
13474 PTMScan® Asymmetric Di-Methyl Arginine Motif [adme-R] Kit Trypsin* Methylation Mouse Liver
XLS  |  RAW 1 2
13563 PTMScan® Symmetric Di-Methyl Arginine Motif [sdme-RG] Kit Trypsin* Methylation Mouse Liver
XLS  |  RAW 1 2
12810 PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Kit Trypsin Cleaved Mouse Liver
XLS  |  RAW 1 2
12170 PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW 1 2
14809 PTMScan® Pan-Methyl Lysine Kit Trypsin* Methylation Mouse Liver
XLS  |  RAW 1 2
14990 PTMScan® Phospho-MAPK Substrate Motif [PXpTP] Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW 1 2
4652 PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P and S*PXK/R) Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW 1 2
5565 PTMScan® Phospho-PKA Substrate Motif (RRXS*/T*) Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW 1 2
14989 PTMScan® Phospho-Ser-Pro-Pro Motif [pSPP] Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW 1 2
5636 PTMScan® Phospho-Tyrosine Mouse mAb (P-Tyr-100) Kit Trypsin Phosphorylation Mouse Brain
XLS  |  RAW
8803 PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit Trypsin Phosphorylation Mouse Liver | XLS | RAW 1 2
14478 PTMScan® Pilot Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Trypsin Phosphorylation Mouse Liver | XLS | RAW 1 2
5567 PTMScan® Phospho-T*PP Motif (T*PP) XP® Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW 1 2
5566 PTMScan® Phospho-ST*P Motif (ST*P) XP® Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW 1 2
13764 PTMScan® Succinyl-Lysine Motif [Succ-K] Kit Trypsin Succinylation Mouse Liver
XLS  |  RAW 1 2
5562 PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit Trypsin Ubiquitin Mouse Liver
XLS  |  RAW 1 2
14482 PTMScan® Pilot Ubiquitin Remnant Motif (K-ε-GG) Kit Trypsin Ubiquitin Mouse Liver
XLS  |  RAW 1 2
32948 PTMScan® Basophilic Kinase Substrate Motif [(R/X)(R/X)Xp(S/T)] Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW 1 2
28303 PTMScan® Phospho-Ser/Thr-Pro Motif [p(S/T)P] Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW 1 2
25081 PTMScan® Phospho-Ser/Thr Motif [pS/T] Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW 1 2
17848 PTMScan® Propionyl-Lysine [Prop-K] Kit Trypsin Propionylation Mouse Liver
XLS  |  RAW 1 2
26101 PTMScan® Glutaryl-Lysine [Glut-K] Kit Trypsin Glutarylation Mouse Liver
XLS  |  RAW 1 2
93872 PTMScan® Malonyl-Lysine [Mal-K] Kit Trypsin Malonylation Mouse Liver
XLS  |  RAW 1 2
10821 PTMScan® Phospho-PDK1 Docking Motif [(F/Y)p(S/T)(F/Y)] Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW 1 2
91336 PTMScan® Phospho-Thr-X-Arg Motif [pT(P/X)R] Kit Trypsin Phosphorylation HT29 Cells
XLS
71652 PTMScan® Phospho-PKC Substrate Motif [(R/K)(R/K)Xp(S/T)X(R/X)] Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW 1 2
Mouse Liver [pT(P/X)R]
XLS  |  RAW 1 2
41343 PTMScan® Anti-Biotin Kit Trypsin Biotinylation β2AR-APEX 293 cells
XLS  |  RAW 1 2 3 4
92465 PTMScan® Sumoylation Remnant Motif Kit WALP SUMOylation Hela cells
XLS  |  RAW 1 2
80660 PTMScan® Sumoylation Remnant Motif Pilot Kit WALP SUMOylation Hela cells
XLS  |  RAW 1 2

* Recommended to perform a secondary trypsin digest post-IP

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