Western Blot (Immunoblot)

Western blotting is a widely used technique to detect levels of protein expression in cell or tissue extract. This technique measures protein levels in a biological sample through antibody binding to a specific protein of interest.

Our rigorous validation protocols help us provide specific and reliable antibodies, which helps you get reproducible and meaningful data every time.

Companion Products for WB

We offer a comprehensive list of companion products that are validated in-house with our protocols so that you can get all of the reagents you need to complete your experiment.

Companion Products for Western Blotting:

Akt Western Blotting Data

Western blot of extracts from various cell lines, untreated or treated as indicated with LY294002 #9901 (a PI3 kinase inhibitor), Human Insulin-like Growth Factor I (hIGF-I) #8917 (which activates Akt via IGFIR binding), or Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 (which induces the PI3K/Akt pathway through receptor binding), using Phospho-Akt (Ser473) (D9E) XP^® Rabbit mAb #4060 (left) and Akt (pan) (C67E7) Rabbit mAb #4691 (right).

Western Blot (Immunoblot)

Companion Products for WB

Companion Products for Western Blotting:

Akt Western Blotting Data

Resources