Interested in promotions? | Click here >>
15020
DAPT
Activators & Inhibitors
Chemical Modulators

DAPT #15020

Reviews ()
Citations (0)
DAPT: Image 1

Chemical structure of DAPT.

DAPT: Image 2

Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with DAPT (24 hr) at the indicated concentrations, using Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147 (upper), Notch1 (D6F11) XP® Rabbit mAb #4380 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Product Usage Information

DAPT is supplied as a lyophilized powder. For a 25 mM stock, reconstitute 5 mg in 462.43 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but are typically used at 10-50 μM for 12-48 hr, respectively.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 432.5 g/mol
Purity

>95%

Molecular Formula

C23H26F2N2O4

CAS 208255-80-5
Solubility Soluble in DMSO at 15mg/ml and EtOH at 1mg/ml.

Background

DAPT is a potent γ-secretase inhibitor. Research studies have demonstrated that DAPT inhibits production of total β-amyloid peptide (Aβ) and Aβ42 in human primary neuronal cultures with an IC50 of 115 nM and 200 nM, respectively (1). DAPT-induced inhibition of Notch1 signaling prevents cleavage formation of the Notch1 intracellular domain (NICD), resulting in down regulation of target gene transcription (2). DAPT treatment has been shown to induce apoptosis in Jurkat (2) and lung squamous cell carcinoma (3) cell lines.

  1. Dovey, H.F. et al. (2001) J Neurochem 76, 173-81.
  2. Luo, X. et al. (2013) Cancer Cell Int 13, 34.
  3. Cao, H. et al. (2012) APMIS 120, 441-50.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
To Purchase # 15020S
Product # Size Price
15020S
5 mg N/A

Powered by Translations.com GlobalLink OneLink SoftwarePowered By OneLink