The working concentration of BMP-2 generally ranges from 20-50 ng/ml.
Lyophilized product is very stable at -20°C. It is recommended to reconstitute with 10 mM HCl at a concentration of 0.1 mg/ml, which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
|Activity||The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml.|
Recombinant human BMP-2 was expressed in E. coli and is supplied in a lyophilized form.
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