Buy 3 and get the 4th for FREE | Learn More >>
1225
Bcl-xL Blocking Peptide
Experimental Controls

Bcl-xL Blocking Peptide #1225

Reviews ()
Citations (0)

Product Usage Information

Use as a blocking reagent to evaluate the specificity of antibody reactivity in western immunoblotting and dot blot protocols. For western blotting, add an equal volume of peptide as volume of antibody used in 10 ml total volume, and incubate at room temperature for 30 minutes before allowing to react with the blot. Recommended antibody dilutions can be found on the Bcl-xL Antibody #2762 and Bcl-xL (54H6) Rabbit mAb #2764 data sheets.

Storage:

Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. 1% DMSO. Store at –20°C.

Product Description

This peptide is used to block Bcl-xL (54H6) Rabbit mAb #2764 and Bcl-xL Antibody #2762 reactivity.

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Bcl-xL (54H6) Rabbit mAb #2764 and Bcl-xL Antibody #2762 signal in both dot blot and western blotting.

Background

Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).

  1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-6.
  2. Minn, A.J. et al. (1999) EMBO J 18, 632-43.
  3. Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9.
  4. Fan, M. et al. (2000) J Biol Chem 275, 29980-5.
  5. Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Important Ordering Details

Custom Ordering Details: This product is assembled upon order. Please allow up to three weeks for your product to be processed.
Powered By OneLink