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9243
NF-κB Control Cell Extracts
Experimental Controls
Cell Extract Kit

NF-κB Control Cell Extracts #9243

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  1. WB
Western Blotting Image 1: NF-κB Control Cell Extracts

Western blot analysis of NF-κB Control Cell Extracts #9243, using IKKα Antibody #2682 (upper), IKKβ (2C8) Rabbit mAb #2370 (middle) and Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697 (lower).

Western Blotting Image 2: NF-κB Control Cell Extracts

Western blot analysis of NF-κB Control Cell Extracts #9243, using IκBα (44D4) Rabbit mAb #4812 (left) and Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 (right).

Western Blotting Image 3: NF-κB Control Cell Extracts

Western blot analysis of NF-κB Control Cell Extracts #9243, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (left) and NF-κB p65 (E498) Antibody #3987 (right).

Product Usage Information

Boil for 3 minutes prior to use. Load 20 µl of untreated and hTNF-α treated NF-κB Control Cell Extracts per lane.

Storage

Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Store at –20°C or –80°C for long-term storage.

Product Description

NF-κB Control Cell Extracts (HeLa untreated): Total cell extracts from HeLa cells serve as a negative control. Supplied SDS Sample Buffer.

NF-κB Control Cell Extracts (HeLa + hTNF-alpha): Total cell extracts from HeLa cells treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 serve as a positive control. Supplied SDS Sample Buffer.

Background

Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).

  1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
  2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
  3. Haskill, S. et al. (1991) Cell 65, 1281-9.
  4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
  5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
  6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
  7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci U S A 92, 11259-63.
  8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
  9. Senftleben, U. et al. (2001) Science 293, 1495-9.
  10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
  11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 9243S
Product # Size Price
9243S
200 µl  (10 western blots) N/A

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