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9769
14-3-3 Family Antibody Sampler Kit
Primary Antibodies

14-3-3 Family Antibody Sampler Kit #9769

Western Blotting Image 1

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 β/α Antibody.

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from various cell types using 14-3-3 ε Antibody.

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Western Blotting Image 5

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody.

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Western Blotting Image 6

Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb.

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Western Blotting Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Isoform Specificity Image 8

Isoform specificity of 14-3-3γ (D15B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each)were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 γ (D15B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower).

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody.

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Isoform Specificity Image 10

Isoform specificity of 14-3-3 η (D23B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each) were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 η (D23B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower).

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded human GIST, using 14-3-3 tau Antibody.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using 14-3-3 tau Antibody.

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
14-3-3 β/α Antibody 9636 20 µl
  • WB
H M R Mk 28 Rabbit 
14-3-3 γ (D15B7) Rabbit mAb 5522 20 µl
  • WB
H M R Mk Pg 27 Rabbit IgG
14-3-3 ζ/δ (D7H5) Rabbit mAb 7413 20 µl
  • WB
H M R Mk Pg 28 Rabbit IgG
14-3-3 ε Antibody 9635 20 µl
  • WB
H M R Mk 28 Rabbit 
14-3-3 τ Antibody 9638 20 µl
  • WB
  • IHC
H M R Mk 28 Rabbit 
14-3-3 η (D23B7) Rabbit mAb 5521 20 µl
  • WB
H M R Mk B Pg 27 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The 14-3-3 Family Antibody Sampler Kit provides an economical means to investigate the expression of various 14-3-3 isoforms within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Each antibody in the 14-3-3 Family Antibody Sampler Kit detects endogenous levels of its respective target.

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequences of human 14-3-3 ß/α, 14-3-3 ε and 14-3-3 τ proteins. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg80 of human 14-3-3 ζ/δ protein, Leu37 of human 14-3-3 η protein and Ile79 of human 14-3-3 γ protein.

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).

  1. Muslin, A.J. and Xing, H. (2000) Cell Signal 12, 703-9.
  2. Mackintosh, C. (2004) Biochem J 381, 329-42.
  3. Dougherty, M.K. and Morrison, D.K. (2004) J Cell Sci 117, 1875-84.
  4. Yaffe, M.B. (2002) FEBS Lett 513, 53-7.
  5. Bridges, D. and Moorhead, G.B. (2004) Sci STKE 2004, re10.
Entrez-Gene Id
7529 , 7531 , 7533 , 7532 , 10971 , 7534
Swiss-Prot Acc.
P31946 , P62258 , Q04917 , P61981 , P27348 , P63104
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Product # Size Price
9769T
1 Kit  (6 x 20 µl) N/A

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