Buy Three, Get the Fourth Free! | Start Saving >>
29087
PhosphoPlus® α-Synuclein (Ser129) Antibody Duet
Primary Antibodies

PhosphoPlus® α-Synuclein (Ser129) Antibody Duet #29087

Western Blotting Image 1

Western blot analysis of extracts from mouse brain, untreated (-) or λ phosphatase-treated (+), using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper) or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower).

Learn more about how we get our images
Western Blotting Image 2

Western blot analysis of extracts from mouse and rat brain using α-Synuclein (D37A6) XP® Rabbit mAb.

Learn more about how we get our images
IF-F Image 3

Confocal immunofluorescent analysis of mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper), or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower) (green), in the presence of Phospho-α-Synuclein (Ser129) peptide (middle) or Non-phospho-α-Synuclein (Ser129) peptide (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Learn more about how we get our images
IHC-P (paraffin) Image 4

Immunohistochemical analysis of paraffin-embedded mouse brain using α-Synuclein (D37A6) XP® Rabbit mAb.

Learn more about how we get our images
IF-F Image 5

Confocal immunofluorescent analysis of untreated (left) or λ phosphatase-treated (right) mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper), or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Learn more about how we get our images
IF-F Image 6

Confocal immunofluorescent analysis of normal rat cerebellum, hippocampus and striatum using α-Synuclein (D37A6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Learn more about how we get our images
IF-F Image 7

Confocal immunofluorescent analysis of mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (green). High-magnification showing punctate and sparse nuclear labeling (arrows). Specificity of nuclear staining is unclear (see Specificity/Sensitivity statement and supporting IF-F validation data). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Learn more about how we get our images
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb 23706 100 µl
  • WB
  • IP
  • IF
H M R 18 Rabbit IgG
α-Synuclein (D37A6) XP® Rabbit mAb 4179 100 µl
  • WB
  • IP
  • IHC
  • IF
M R 18 Rabbit IgG

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-synuclein gene are linked to Parkinson's disease (1).

Various research studies have shown that phosphorylation of α-Synuclein at Ser129 is a highly toxic event that causes degeneration of dopaminergic neurons, which are associated with Parkinson's disease. This is proposed to occur through increased misfolding, aggregation, and accumulation of α-Synuclein phosphorylated at this site (2). GSK-3β is one of several kinases that has been reported to phosphorylate α-Synuclein at Ser129 (3).

  1. Goldberg, M.S. and Lansbury, P.T. (2000) Nat Cell Biol 2, E115-9.
  2. Oueslati, A. et al. (2010) Prog Brain Res 183, 115-45.
  3. Credle, J.J. et al. (2015) Cell Death Differ 22, 838-51.
  4. Yu, S. et al. (2007) Neuroscience 145, 539-55.
Entrez-Gene Id
6622 , 20617
Swiss-Prot Acc.
P37840 , O55042
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Your Local Representative for the
United Kingdom

New England Biolabs (UK) Ltd.

75-77 Knowl Piece Wilbury Way

Hitchin, Hertfordshire

Phone:
+44 1462 420 616
Phone:
0800 318486 [Toll Free]
Fax:
+44 1462 421 057
Fax:
0800 435682 [Toll Free]
Email:
info.uk@neb.com
URL:
www.neb.uk.com

Need information for a different country? Please click here.

To get local purchase information on this product, click here.

Powered By OneLink