Western blot analysis of extracts from various cell lines using APOBEC3B (E9A2G) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
APOBEC3B (E9A2G) Rabbit mAb recognizes endogenous levels of total APOBEC3B protein. This antibody weakly recognizes a 50 kDa band on unknown origen in cell lysates. Based on the sequence of the peptide antigen, this antibody is not expected to cross-react with other APOBEC3 family members.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro206 of human APOBEC3B protein.
Members of the APOBEC3 subfamily of cytosine deaminases (APOBEC3A, APOBEC3B, APOBEC3C, APOBEC3D, APOBEC3F, APOBEC3G, and APOBEC3H) function in innate immunity by preventing viral DNA replication, including that of HIV-1 (1-2). APOBEC3B mutation and aberrant expression in cancer is thought to result in mutation of genomic DNA, and to drive tumorigenesis. APOBEC3B is highly expressed in human breast cancer, glioma, and other human cancers (3-5).
Explore pathways + proteins related to this product.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.