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74437
Fanconi Anemia Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Fanconi Anemia Antibody Sampler Kit #74437

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Fanconi Anemia Antibody Sampler Kit: Image 1

Immunoprecipitation of FANCA from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or FANCA (D1L2Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using FANCA (D1L2Z) Rabbit mAb.

Fanconi Anemia Antibody Sampler Kit: Image 2

Western blot analysis of extracts from various cell types using FANCB (D9W6S) Rabbit mAb.

Fanconi Anemia Antibody Sampler Kit: Image 3

Immunoprecipitation of FANCD2 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is FANCD2 (D5L5X) Rabbit mAb. Western blot analysis was performed using FANCD2 (D5L5X) Rabbit mAb.

Fanconi Anemia Antibody Sampler Kit: Image 4

Western blot analysis of extracts from various cell lines using BRCA2 (D9S6V) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Fanconi Anemia Antibody Sampler Kit: Image 5

Western blot analysis of extracts from SH-SY5Y, Saos-2 and Jurkat cells using BACH1/BRIP1 Antibody.

Fanconi Anemia Antibody Sampler Kit: Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Fanconi Anemia Antibody Sampler Kit: Image 7

Western blot analysis of extracts from various cell lines using FANCA (D1L2Z) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, GM06914 cells are negative for FANCA.

Fanconi Anemia Antibody Sampler Kit: Image 8

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human FANCB (FANCB-Myc/DDK; +) protein, using FANCB (D9W6S) Rabbit mAb.

Fanconi Anemia Antibody Sampler Kit: Image 9

Western blot analysis of extracts from HeLa, U-2 OS, and GM16633 (PD20, FANCD2-negative) cells using FANCD2 (D5L5X) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Fanconi Anemia Antibody Sampler Kit: Image 10

Western blot analysis of DLD1 cells, either wild type (WT) or BRCA2 knockout (-/-), using BRCA2 (D9S6V) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb (lower). DLD1 (WT) and DLD1 (-/-) cells were kindly provided by Dr. Judit Jimenez, Yale University, New Haven, CT.

Fanconi Anemia Antibody Sampler Kit: Image 11

Western blot analysis of extracts from HeLa cells, untreated or treated with mitomycin C (2 μg/mL, 24 hr), using FANCD2 (D5L5X) Rabbit mAb. DNA damage caused by mitomycin C induces monoubiquitination of FANCD2, altering its electrophoretic mobility and increasing its apparent molecular weight (4).

To Purchase # 74437T
Product # Size Price
74437T
1 Kit  (5 x 20 µl) N/A

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
FANCA (D1L2Z) Rabbit mAb 14657 20 µl
  • WB
  • IP
H 160 Rabbit IgG
FANCB (D9W6S) Rabbit mAb 14243 20 µl
  • WB
H M R 90 Rabbit IgG
FANCD2 (D5L5X) Rabbit mAb 16323 20 µl
  • WB
  • IP
H 155, 162 Rabbit IgG
BRCA2 (D9S6V) Rabbit mAb 10741 20 µl
  • WB
H 380 Rabbit IgG
BACH1/BRIP1 Antibody 4578 20 µl
  • WB
H 145 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Fanconi Anemia Antibody Sampler Kit provides an economical means of detecting members of the Fanconi Anemia signaling pathway. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Fanconi Anemia Antibody Sampler kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Ala514 of human FANCA, Pro280 of human FANCB, Gly995 of human FANCD2, and a recombinant protein specific to the carboxy terminus of human BRCA2. Polyclonal BACH1/BRIP1 Antibody is produced by immunizing rabbits with a synthetic peptide corresponding to amino acids near the carboxy terminus of human BACH1. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.

Background

Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). In response to DNA damage, the FA nuclear complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCM) induces mono-ubiquitination of FANCD2 and FANCI (2).

Monoubiquitination of FANCD2 induces localization of FANCD2 to sites of DNA damage, where it interacts with BRCA1 (4). FANCJ/BRIP1, FANCD1/BRCA2, and FANCN/PALB2 are also recruited to sites of DNA damage. FA signaling is important in maintenance of chromosome stability and control of mitosis (3).

  1. Alter, B.P. (1996) Am J Hematol 53, 99-110.
  2. Fei, P. et al. (2005) Cell Cycle 4, 80-6.
  3. Nalepa, G. and Clapp, D.W. (2014) F1000Prime Rep 6, 23.
  4. Garcia-Higuera, I. et al. (2001) Mol Cell 7, 249-62.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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