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63815
PD-1 (D4W2J) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

PD-1 (D4W2J) XP® Rabbit mAb (BSA and Azide Free) #63815

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  1. WB
  2. IHC
Western Blotting Image 1: PD-1 (D4W2J) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from human CD4+ T cells, MOLT-4, and Jurkat cells using PD-1 (D4W2J) XP® Rabbit mAb (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of human interleukin-2 (hIL-2) #8907 (6.7 ng/ml). Data was generated using the standard formulation of this product.
Immunohistochemistry Image 1: PD-1 (D4W2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using PD-1 (D4W2J) XP® Rabbit mAb. Data was generated using the standard formulation of this product.
Immunohistochemistry Image 2: PD-1 (D4W2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PD-1 (D4W2J) XP® Rabbit mAb. Data was generated using the standard formulation of this product.
Immunohistochemistry Image 3: PD-1 (D4W2J) XP® Rabbit mAb (BSA and Azide Free)
Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using PD-1 (D4W2J) XP® Rabbit mAb (green), B7-H3 (D9M2L) XP® Rabbit mAb #14058 (red), B7-H4 (D1M8I) XP® Rabbit mAb (cyan), LAG3 (D2G4O) XP® Rabbit mAb #15372 (magenta), TIM-3 (D5D5R) XP® Rabbit mAb #45208 (yellow), and VISTA (D1L2G) XP® Rabbit mAb #64953 (orange). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: PD-1 (D4W2J) XP® Rabbit mAb (BSA and Azide Free)
Multiplex immunohistochemical analysis of paraffin-embedded human breast carcinoma usng PD-1 (D4W2J) XP® Rabbit mAb (green), PD-L1 (E1L3N®) XP® Rabbit mAb #13684 (red), LAG3 (D2G4O) XP® Rabbit mAb #15372 (magenta), TIM-3 (D5D5R) XP® Rabbit mAb #45208 (yellow), CD8α (C8/144B) Mouse mAb #70306 (orange), and Pan-keratin (C11) Mouse mAb #4545 (cyan). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: PD-1 (D4W2J) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded 293 cell pellets, control (left) or PD-1 transfected (right), using PD-1 (D4W2J) XP® Rabbit mAb. Data was generated using the standard formulation of this product.
To Purchase # 63815
Cat. # Size Qty. Price
63815SF
100 µg N/A

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 52-65
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #86163. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #86163.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

PD-1 (D4W2J) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total PD-1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala274 of human PD-1 protein.

Background

The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T cells, PD-1 is expressed in activated B cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).
  1. Ishida, Y. et al. (1992) EMBO J 11, 3887-95.
  2. Shinohara, T. et al. (1994) Genomics 23, 704-6.
  3. Nishimura, H. et al. (1999) Immunity 11, 141-51.
  4. Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
  5. Yokosuka, T. et al. (2012) J Exp Med 209, 1201-17.
  6. Sheppard, K.A. et al. (2004) FEBS Lett 574, 37-41.
  7. Chemnitz, J.M. et al. (2004) J Immunol 173, 945-54.
  8. Thibult, M.L. et al. (2013) Int Immunol 25, 129-37.
  9. Dong, H. et al. (2002) Nat Med 8, 793-800.
  10. Topalian, S.L. et al. (2012) Curr Opin Immunol 24, 207-12.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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