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9968
Rho-GTPase Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Rho-GTPase Antibody Sampler Kit #9968

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Rho-GTPase Antibody Sampler Kit: Image 1

Western blot analysis of extracts from various cell lysates, using Cdc42 (11A11) Rabbit mAb.

Rho-GTPase Antibody Sampler Kit: Image 2

Western blot analysis of A431 cell extracts, untreated (-) or EGF treated (+), using Phospho-Rac1/cdc42 (Ser71) Antibody (upper) or Rac1/Cdc42 Antibody #4651 (lower).

Rho-GTPase Antibody Sampler Kit: Image 3

Western blot analysis of extracts from various cell lines, using RhoA (67B9) Rabbit mAb.

Rho-GTPase Antibody Sampler Kit: Image 4

Western blot analysis of extracts from mouse and rat brain tissue using RhoB Antibody.

Rho-GTPase Antibody Sampler Kit: Image 5

Western blot analysis of extracts from various cell types using RhoC (D40E4) Rabbit mAb.

Rho-GTPase Antibody Sampler Kit: Image 6

Flow cytometric analysis of Jurkat cells, using Rac1/2/3 Antibody (blue) compared to a nonspecific negative control antibody (red).

Rho-GTPase Antibody Sampler Kit: Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Rho-GTPase Antibody Sampler Kit: Image 8

Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoA (67B9) Rabbit mAb.

Rho-GTPase Antibody Sampler Kit: Image 9

Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoB Antibody.

Rho-GTPase Antibody Sampler Kit: Image 10

Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoC (D40E4) Rabbit mAb.

Rho-GTPase Antibody Sampler Kit: Image 11

Western blot analysis of extracts from COS, C6, NIH/3T3 and HEK293 cells, using Rac1/2/3 Antibody.

To Purchase # 9968T
Product # Size Price
9968T
1 Kit  (6 x 20 µl) N/A

Kit Usage Information

Protocols

Product Description

The Rho-GTPase Antibody Sampler Kit contains reagents to examine aspects of cell migration, adhesion, proliferation and differentiation in cells. This kit includes enough primary and secondary antibodies to perform two Western blot experiments per each primary antibody.

Specificity / Sensitivity

Cdc42 (11A11) Rabbit mAb detects endogenous levels of total Cdc42 protein and does not cross-react with other small GTPases. Phospho-Rac1/cdc42 (Ser71) Antibody detects endogenous Rac1/cdc42 only when phosphorylated at Ser71 and may also recognize phospho-RhoA (Ser73). Rac 1/2/3 Antibody detects endogenous levels of total Rac1/2/3 proteins. RhoA (67B9) Rabbit mAb recognizes endogenous levels of total RhoA protein. RhoB Antibody recongnizes endogenous levels of RhoB. RhoC (D40E4) XP® Rabbit mAb recongnizes endogenous levels of RhoC.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr138 of human Rac1, corresponding to residues surrounding Ser71 of human Rac1/cdc42, and corresponding to residues near the carboxy terminus of RhoB . Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding Lys135 of human Cdc42, residues near the carboxy terminus of human RhoA, and to residues corresponding to the carboxy terminus of human RhoC.

Background

The Rho family of small GTPases, including Rho, Rac, and cdc42, act as molecular switches to regulate processes such as cell migration, adhesion, proliferation and differentiation (1). RhoA, RhoB and RhoC are all highly homologous but appear to have divergent biological functions. The best characterized of these proteins, RhoA, regulates acomysin contractility, cytokinesis, focal adhesion assembly and cell polarity (2-5). Mammalian Rac exists as three isoforms (Rac1, Rac2 and Rac3) that show high sequence similarity. Well-characterized Rac1 and cdc42 are ubiquitously expressed and play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, and cell growth and development (6). Phosphorylation of Rac1 at a putative Akt site (Ser71) may limit Rac1 activity through inhibition of GTP binding (7). Rac2 is expressed in cells of hematopoietic origin, while Rac3 is highly expressed in brain and in many other tissues. The Vav family of guanine-nucleotide exchange factors mediates activation of Rho/Rac family small GTPases (8). Negative regulation of Rho-activity members of the p190 RhoGAP family (p190-A and p190-B) may be controlled by Src phosphorylation of Tyr residues, activating the p190 GAP domain (8-10). Furthermore, Rho GDP dissociation inhibitor (RhoGDI) associates with Rho/Rac to negatively regulate nucleotide exchange membrane localization (11).

  1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol. 15, 356-363.
  2. Bi, D. et al. (2005) Circ. Res. 96, 890-897.
  3. Kimura, K. et al. (2000) J. Biol. Chem. 275, 17233-17236.
  4. Barry, S.T. and Critchley, D.R. (1994) J. Cell Sci. 107 ( Pt 7), 2033-2045.
  5. Van Keymeulen, A. et al. (2006) J. Cell Biol. 174, 437-445.
  6. Wennerberg, K. and Der, C.J. (2004) J. Cell Sci. 117, 1301-1312.
  7. Kwon, T. et al. (2000) J. Biol. Chem. 275, 423-428.
  8. Sordella, R. et al. (2003) Cell 113, 147-158.
  9. Chang, J.H. et al. (1995) J. Cell Biol. 130, 355-368.
  10. Roof, R.W. et al. (1998) Mol. Cell Biol. 18, 7052-7063.
  11. Dovas, A. and Couchman, J.R. (2005) Biochem. J. 390, 1-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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