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8887
Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate)
Secondary Antibodies

Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8887

APPLICATIONS

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (PE Conjugate) (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red).

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The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.

Storage:

Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Anti-mouse IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.

This product has been optimized for use as a secondary antibody in FLOW ctytometry. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Product # Size
8887S
250 µl

Your Local Representative for the
United Kingdom

New England Biolabs (UK) Ltd.

75-77 Knowl Piece Wilbury Way

Hitchin, Hertfordshire

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+44 1462 420 616
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0800 318486 [Toll Free]
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+44 1462 421 057
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0800 435682 [Toll Free]
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