Store at room temperature in a cool and dry place away from light or heat sources. Nitrocellulose is extremely flammable and must be kept away from fire to avoid ignition. Stored appropriately, the product is stable for 12 months. Handle with gloves; nitrocellulose is highly absorbent and will bind proteins from skin contact which may reduce performance and decrease the quality of data.
Nitrocellulose Membrane Dimensions: 80 x 90 mm, Pore Size: 0.2 μm, Binding Capacity: 115-125 μg IgG/cm2
100% pure nitrocellulose.
Nitrocellulose Sandwiches from Cell Signaling Technology® are pre-packaged sandwiches consisting of a precut nitrocellulose membrane and 2 sheets of 3 mm thick blotting paper. Intended to provide convenience and time savings, they are also validated for use in CST's Western Blot protocol and are the preferred membrane used by Cell Signaling Technology scientists.
Nitrocellulose Sandwiches are ideal for binding proteins of all molecular weights; the 0.2 μm pore size ensures high retention of low molecular weight proteins, even below 20 kDa. Nitrocellulose Sandwiches are suitable for most common transfer methods including tank, semi-dry, and vacuum blotting. This product is also suitable for colorimetric, fluorescent, and chemiluminescent detection. For chemiluminescent detection, CST recommends HRP based systems. Unlike PVDF, nitrocellulose does not require a methanol pre-wetting step.